Vitamin A deficiency and associated risk factors in children aged 12-59 months living in poorest municipalities in the South Region of Brazil.

OBJECTIVE: To estimate the prevalence of vitamin A deficiency (VAD) in children and associated risk factors. DESIGN: Analysis of data from a cross-sectional multicentre study performed in the primary care units of the municipalities from January to June 2015. The children's legal guardians answered a socio-economic questionnaire, and the children's blood samples were obtained by venipuncture. Plasma retinol was determined by HPLC. Plasma retinol values of <0·70 µmol/l were considered VDA. Poisson multiple regression with robust variance was used. Values of P < 0·05 were considered significant. The data were analysed in the SPSS software, 21.0. SETTING: Forty-eight poorest municipalities in the South Region of Brazil. PARTICIPANTS: Children (n 1503) aged 12-59 months. RESULTS: The prevalence of VAD in the sample was 1·9 % (95 % CI (0·5, 6·8)). The following risk factors were associated with the outcome in the final explanatory model: family received Bolsa Familia program benefits (PR = 3·19; 95 % CI (1·69, 6·02)), child was not being breastfed (PR = 5·22; 95 % CI (1·68, 16·18)) and stunting (PR = 4·75; 95 % CI (2·10, 10·73)). CONCLUSIONS: VAD did not represent a public health problem for children living in socio-economically vulnerable municipalities in the South Region of Brazil, suggesting a new panorama of this nutritional deficiency even in regions of low socio-economic conditions in these three states. Thus, in view of the current nutritional transition scenario, it is necessary to continuously monitor and improve public policies related to vitamin A supplementation in the country.

Individual and contextual predictors of children's hemoglobin levels from Southern Brazilian municipalities in social vulnerability.

Few studies have investigated the simultaneous effect of individual and contextual factors on the occurrences of anemia. This study aims to evaluate the variability of children's hemoglobin levels from municipalities in social vulnerability and its association with factors of individual and municipal nature. This is a cross-sectional, multi-center study, with children data (12-59 months) collected from 48 municipalities of the Southern region of Brazil, that were included in the Brazil Without Poverty Plan. Individuals' data were collected using a structured questionnaire, and secondary and ecological data of children's municipalities were collected via national surveys and institutional websites. The outcome was defined as the hemoglobin level obtained by HemoCue. A multilevel analysis was performed using Generalized Linear Models for Location Scale and Shape using R, with a 5% significance level. A total of 1,501 children were evaluated. The mean hemoglobin level was 12.8g/dL (95%CI: 12.7-12.8), with significant variability between municipalities. Lower values of hemoglobin were observed in children who lived in municipalities with a higher urbanization rate and a lower number of Community Health Agents, in relation to the reference categories. At the individual level, lower hemoglobin values were identified for children under 24 months, not enrolled at daycares, who were beneficiaries of the conditional cash transfer program and diagnosed with underweight. The results shed light on important factors at the municipal and the individual levels that were associated to the hemoglobin levels of children living in municipalities in social vulnerability.

Individual and contextual predictors of children's hemoglobin levels from Southern Brazilian municipalities in social vulnerability / Preditores individuais e contextuais dos níveis de hemoglobina em crianças de municípios do Sul do Brasil com vulnerabilidade social / Predictores individuales y contextuales de los niveles de hemoglobina en niños socialmente vulnerables de municipios del Sur de Brasil

Abstract: Few studies have investigated the simultaneous effect of individual and contextual factors on the occurrences of anemia. This study aims to evaluate the variability of children's hemoglobin levels from municipalities in social vulnerability and its association with factors of individual and municipal nature. This is a cross-sectional, multi-center study, with children data (12-59 months) collected from 48 municipalities of the Southern region of Brazil, that were included in the Brazil Without Poverty Plan. Individuals' data were collected using a structured questionnaire, and secondary and ecological data of children's municipalities were collected via national surveys and institutional websites. The outcome was defined as the hemoglobin level obtained by HemoCue. A multilevel analysis was performed using Generalized Linear Models for Location Scale and Shape using R, with a 5% significance level. A total of 1,501 children were evaluated. The mean hemoglobin level was 12.8g/dL (95%CI: 12.7-12.8), with significant variability between municipalities. Lower values of hemoglobin were observed in children who lived in municipalities with a higher urbanization rate and a lower number of Community Health Agents, in relation to the reference categories. At the individual level, lower hemoglobin values were identified for children under 24 months, not enrolled at daycares, who were beneficiaries of the conditional cash transfer program and diagnosed with underweight. The results shed light on important factors at the municipal and the individual levels that were associated to the hemoglobin levels of children living in municipalities in social vulnerability.

Resumo: Poucos estudos investigaram o efeito simultâneo dos fatores individuais e contextuais sobre a ocorrência da anemia. O estudo procura avaliar a variabilidade dos níveis de hemoglobina em crianças de municípios com vulnerabilidade social e a associação com fatores individuais e municipais. Foi realizado um estudo transversal com dados de crianças de idade pré-escolar (12-59 meses) de um estudo multicêntrico em 48 municípios do Sul do Brasil, incluídos no Plano Brasil Sem Miséria. Os dados dos indivíduos foram coletados com um questionário estruturado, e os dados secundários e ecológicos dos municípios das crianças foram obtidos através de inquéritos nacionais e websites institucionais. O desfecho foi definido como o nível de hemoglobina, obtido com o sistema HemoCue. Foi realizada análise multinível usando modelos lineares generalizados para posição, escala e forma, no R, com nível de 5% de significância . Foram avaliadas 1.501 crianças. O nível médio de hemoglobina foi 12,8g/dL (IC95%: 12,7-12,8), com variabilidade significativa entre os municípios. Níveis de hemoglobina mais baixos foram observados nas crianças em municípios com taxas de urbanização mais altas e menor número de agentes comunitários de saúde, comparado com as categorias de referência. Em nível individual, níveis de hemoglobina mais baixos foram identificados em crianças abaixo de 24 meses, não matriculadas em creches, beneficiárias do Programa Bolsa Família e diagnosticadas com baixo peso. Os resultados destacam fatores importantes nos níveis municipal e individual que estão associados com os níveis de hemoglobina em crianças de municípios com vulnerabilidade social.

Resumen: Pocos estudios han investigado el efecto simultáneo de los factores individuales y contextuales en la incidencia de anemia. El objetivo de este estudio fue evaluar la variabilidad de los niveles de hemoglobina en niños socialmente vulnerables en municipios del sur de Brasil y su asociación con factores en el nivel individual y municipal. Se trata de un estudio trasversal con datos de niños (12-59 meses), procedentes de un estudio multicéntrico, realizado en 48 municipios de la región sur de Brasil, incluidos en el Plan Brasil sin Pobreza. Se recogieron los datos de los participantes, usando un cuestionario estructurado, así como datos secundarios y ecológicos de los municipios de los niños, a través de encuestas nacionales y sitios web institucionales. El resultado se definió como el nivel de hemoglobina obtenido por HemoCue. Se realizó un análisis multinivel, usando modelos lineales generalizados para la escala de localización y forma usando R, con un nivel de un 5% de significancia. Un total de 1.501 niños fueron evaluados. La media de nivel de hemoglobina fue 12,8g/dL (95%CI: 12,7-12,8), con una significativa variabilidad entre municipios. Los valores más bajos de hemoglobina se observaron en niños que vivían en municipios con unas tasas más altas de urbanización, y un número de agentes de salud comunitario más bajo, en relación con las categorías de referencia. En el nivel individual, los valores de hemoglobina más bajos fueron identificados en niños con menos de 24 meses, no matriculados en guarderías, beneficiarios de ayudas económicas, enmarcadas en programas de ayuda económica, y diagnosticados como con bajo peso. Los resultados aclararon importantes factores en el nivel municipal e individual que estaban asociados a los niveles de hemoglobina de niños residentes en municipios, así como vulnerables socialmente.

Introduction of inappropriate complementary feeding in the first year of life and associated factors in children with low socioeconomic status. / Introdução de alimentos não recomendados no primeiro ano de vida e fatores associados em crianças de baixo nível socioeconômico.

The study aimed to identify factors associated with the introduction of inappropriate complementary feeding in the first year of life in children living in municipalities (counties) with low socioeconomic statusl. This was a cross-sectional multicenter study in 1,567 children 12 to 59 months of age in 48 municipalities participating in the Brazil Without Poverty plan in the South of Brazil. A structured questionnaire was applied to the children's parents to obtain socio-demographic information and the age at which inappropriate complementary foods were introduced for the first time in complementary feeding. Prevalence of introduction of sugar before four months of age was 35.5% (n = 497; 95%CI: 33.1-38.0). The prevalence rates for the introduction of cookies/crackers, creamy yogurt, and jelly before six months of age were 20.4% (n = 287; 95%CI: 18.3-22.3), 24.8% (n = 349; 95%CI: 22.4-27.1), and 13.8% (n = 192; 95%CI: 12.0-15.7), respectively. Associations were identified between low maternal schooling (PR = 1.25; 95%CI: 1.03-1.51) and low monthly family income (PR = 1.22; CI95%: 1.01-1.48) and the introduction of inappropriate complementary feeding. The study identified the introduction of inappropriate complementary feeding in the first year of life among children in municipalities with high socioeconomic vulnerability in the South of Brazil, associated with low maternal schooling and low monthly family income.

Identificar os fatores associados à introdução de alimentos não recomendados no primeiro ano de vida, entre crianças residentes em municípios de baixo nível socioeconômico. Estudo multicêntrico transversal com 1.567 crianças de 12 a 59 meses de idade residentes em 48 municípios participantes do plano Brasil Sem Miséria da Região Sul do Brasil. Aplicou-se questionário estruturado aos responsáveis pelas crianças para a obtenção das informações sociodemográficas e idade na qual alimentos não recomendados foram introduzidos pela primeira vez na alimentação complementar. A prevalência de introdução de açúcar antes dos quatro meses de idade da criança foi de 35,5% (n = 497; IC95%: 33,1-38,0). As prevalências de introdução de biscoito doce/salgado, queijo petit suisse e gelatina antes do sexto mês de vida da criança foram de 20,4% (n = 287; IC95%: 18,3-22,3), 24,8% (n = 349; IC95%: 22,4-27,1) e 13,8% (n = 192; IC95%: 12,0-15,7), respectivamente. Identificou-se associação entre a menor escolaridade materna (RP = 1,25; IC95%: 1,03-1,51) e a menor renda mensal familiar (RP = 1,22; IC95%: 1,01-1,48) com a introdução de alimentos não recomendados. Verificou-se a introdução de alimentos não recomendados no primeiro ano de vida entre crianças residentes em municípios de alta vulnerabilidade socioeconômica da Região Sul do Brasil, e esta prática associou-se à menor escolaridade materna e menor renda familiar mensal.

El estudio tuvo como fin identificar los factores asociados a la introducción de alimentos no recomendados durante el primer año de vida, entre niños residentes en municipios con un bajo nivel socioeconómico. Se trata de un estudio multicéntrico transversal con 1.567 niños de 12 a 59 meses de edad, residentes en 48 municipios participantes en el plan Brasil Sin Miseria de la región Sur de Brasil. Se aplicó un cuestionario estructurado a los responsables de los niños para la obtención de la información sociodemográfica y la edad en la que los alimentos no recomendados se introdujeron por primera vez en la alimentación complementaria. La prevalencia de introducción del aúcar, antes de los cuatro meses de edad del niño, fue de un 35,5% (n = 497; IC95%: 33,1-38,0). Las prevalencias de la introducción de galletas dulce/saladas, queso petit suisse y gelatina antes del sexto mes de vida del niño fueron de un 20,4% (n = 287; IC95%: 18,3-22,3), un 24,8% (n = 349; IC95%: 22,4-27,1) y un 13,8% (n = 192; IC95%: 12,0-15,7), respectivamente. Se identificó una asociación entre la menor escolaridad materna (RP = 1,25; IC95%: 1,03-1,51) y la menor renta mensual familiar (RP = 1,22; IC95%: 1,01-1,48), con la introducción de alimentos no recomendados. Se verificó la introducción de alimentos no recomendados durante el primer año de vida entre niños residentes en municipios de alta vulnerabilidad socioeconómica de la región Sur de Brasil, y esta práctica se asoció a una menor escolaridad materna y una menor renta familiar mensual.

Introdução de alimentos não recomendados no primeiro ano de vida e fatores associados em crianças de baixo nível socioeconômico / Introducción de alimentos no recomendados durante el primer año de vida y sus factores asociados en niños con bajo nivel socioeconómico / Introduction of inappropriate complementary feeding in the first year of life and associated factors in children with low socioeconomic status

Identificar os fatores associados à introdução de alimentos não recomendados no primeiro ano de vida, entre crianças residentes em municípios de baixo nível socioeconômico. Estudo multicêntrico transversal com 1.567 crianças de 12 a 59 meses de idade residentes em 48 municípios participantes do plano Brasil Sem Miséria da Região Sul do Brasil. Aplicou-se questionário estruturado aos responsáveis pelas crianças para a obtenção das informações sociodemográficas e idade na qual alimentos não recomendados foram introduzidos pela primeira vez na alimentação complementar. A prevalência de introdução de açúcar antes dos quatro meses de idade da criança foi de 35,5% (n = 497; IC95%: 33,1-38,0). As prevalências de introdução de biscoito doce/salgado, queijo petit suisse e gelatina antes do sexto mês de vida da criança foram de 20,4% (n = 287; IC95%: 18,3-22,3), 24,8% (n = 349; IC95%: 22,4-27,1) e 13,8% (n = 192; IC95%: 12,0-15,7), respectivamente. Identificou-se associação entre a menor escolaridade materna (RP = 1,25; IC95%: 1,03-1,51) e a menor renda mensal familiar (RP = 1,22; IC95%: 1,01-1,48) com a introdução de alimentos não recomendados. Verificou-se a introdução de alimentos não recomendados no primeiro ano de vida entre crianças residentes em municípios de alta vulnerabilidade socioeconômica da Região Sul do Brasil, e esta prática associou-se à menor escolaridade materna e menor renda familiar mensal.

El estudio tuvo como fin identificar los factores asociados a la introducción de alimentos no recomendados durante el primer año de vida, entre niños residentes en municipios con un bajo nivel socioeconómico. Se trata de un estudio multicéntrico transversal con 1.567 niños de 12 a 59 meses de edad, residentes en 48 municipios participantes en el plan Brasil Sin Miseria de la región Sur de Brasil. Se aplicó un cuestionario estructurado a los responsables de los niños para la obtención de la información sociodemográfica y la edad en la que los alimentos no recomendados se introdujeron por primera vez en la alimentación complementaria. La prevalencia de introducción del aúcar, antes de los cuatro meses de edad del niño, fue de un 35,5% (n = 497; IC95%: 33,1-38,0). Las prevalencias de la introducción de galletas dulce/saladas, queso petit suisse y gelatina antes del sexto mes de vida del niño fueron de un 20,4% (n = 287; IC95%: 18,3-22,3), un 24,8% (n = 349; IC95%: 22,4-27,1) y un 13,8% (n = 192; IC95%: 12,0-15,7), respectivamente. Se identificó una asociación entre la menor escolaridad materna (RP = 1,25; IC95%: 1,03-1,51) y la menor renta mensual familiar (RP = 1,22; IC95%: 1,01-1,48), con la introducción de alimentos no recomendados. Se verificó la introducción de alimentos no recomendados durante el primer año de vida entre niños residentes en municipios de alta vulnerabilidad socioeconómica de la región Sur de Brasil, y esta práctica se asoció a una menor escolaridad materna y una menor renta familiar mensual.

The study aimed to identify factors associated with the introduction of inappropriate complementary feeding in the first year of life in children living in municipalities (counties) with low socioeconomic statusl. This was a cross-sectional multicenter study in 1,567 children 12 to 59 months of age in 48 municipalities participating in the Brazil Without Poverty plan in the South of Brazil. A structured questionnaire was applied to the children's parents to obtain socio-demographic information and the age at which inappropriate complementary foods were introduced for the first time in complementary feeding. Prevalence of introduction of sugar before four months of age was 35.5% (n = 497; 95%CI: 33.1-38.0). The prevalence rates for the introduction of cookies/crackers, creamy yogurt, and jelly before six months of age were 20.4% (n = 287; 95%CI: 18.3-22.3), 24.8% (n = 349; 95%CI: 22.4-27.1), and 13.8% (n = 192; 95%CI: 12.0-15.7), respectively. Associations were identified between low maternal schooling (PR = 1.25; 95%CI: 1.03-1.51) and low monthly family income (PR = 1.22; CI95%: 1.01-1.48) and the introduction of inappropriate complementary feeding. The study identified the introduction of inappropriate complementary feeding in the first year of life among children in municipalities with high socioeconomic vulnerability in the South of Brazil, associated with low maternal schooling and low monthly family income.

O perfil epidemiológico da sífilis congênita em uma região de saúde do Rio Grande do Sul, 2015 / The epidemiological profile of congenital syphilis in a health region of Rio Grande do Sul, 2015

A sífilis congênita vem apresentando altas taxas de detecção em todo o país, sendo considerada, portanto, um grave problema de saúde pública. Sendo assim, o objetivo deste trabalho foi descrever o perfil epidemiológico dos casos de sífilis congênita da 10a Região de Saúde do Rio Grande do Sul do ano de 2015. Trata-se de um estudo quantitativo, transversal e descritivo através das variáveis disponíveis nas fichas de notificação do Sistema de Informação. Os dados coletados foram organizados em uma planilha eletrônica, sendo, posteriormente, descritos. Foram identificados 752 casos confirmados e 229 notificações de sífilis em gestante relacionadas com os casos de sífilis congênita. A incidência de sífilis congênita no ano de 2015 na região 10 (23,1/1000 nascidos vivos) foi superior a todas as outras regiões do Estado, inclusive à taxa estadual (11,2/1000 nascidos vivos). Os casos confirmados em sua maioria foram provenientes dos municípios mais populosos, sendo a quase totalidade crianças assintomáticas, nascidas vivas, apresentando falhas de informação no seguimento. A média de idade das mães foi de 25,42 anos (DP ± 6,6), raça/cor branca, baixa escolaridade (< 8 anos de estudo), acesso ao pré-natal e ausência de tratamento do parceiro. A incidência da sífilis congênita na região sugere falhas na implementação de políticas públicas voltadas para a prevenção, dentre elas, a assistência pré-natal e fatores de vulnerabilidade que necessitam de uma rede de assistência articulada. A falta de informações em algumas variáveis mostrou a necessidade constante de qualificação no intuito de conhecer a real situação do agravo na localidade.

Congenital syphilis has been showing high rates of detection throughout the country, and is therefore considered a serious public health problem. Therefore, the objective of this study was to describe the epidemiological profile of congenital syphilis cases of the 10th Health Region of Rio Grande do Sul in the year 2015. This is a quantitative, cross-sectional and descriptive study using the variables available in the notification of the Information System. The data collected were organized in a spreadsheet and later described. A total of 752 confirmed cases and 229 reports of syphilis in pregnant women related to cases of congenital syphilis were identified. The incidence of congenital syphilis in region 10 (23.1/1000 live births) in 2015 was higher than all other regions of the state, including the state rate (11.2/1000 live births). Most confirmed cases came the most populous municipalities, and almost all children were asymptomatic, born alive, presenting information failures at follow-up. The mean age of the mothers was 25.42 years (SD ± 6.6), white race/color, low schooling (<8 years of schooling), access to prenatal care and absence of partner treatment. The incidence of congenital syphilis in the region suggests failures in the implementation of Public Policies aimed at its prevention, among them prenatal care and vulnerability factors that require an articulated care network. The lack of information on some variables showed the constant need for qualification in order to know the real situation of the disease in the locality.

Analysis of the Association of Nonsynonymous Polymorphisms in ADH Genes with Hazardous Drinking in HIV-1-Positive Individuals.

BACKGROUND: Hazardous drinking (HD) is a serious health problem in people infected with human immunodeficiency virus type 1 (HIV-1). Single nucleotide polymorphisms (SNPs) in alcohol dehydrogenase (ADH) genes have been associated with HD in different populations, but there were no data about this in HIV-1-positive individuals. This study investigated the association of 4 nonsynonymous SNPs in ADH genes (Arg48His and Arg370Cys in ADH1B gene; Arg272Gln and Ile350Val in ADH1C gene) with HD in people living with HIV-1. METHODS: This case-control study included 365 HIV-1-positive individuals (121 with HD and 244 without HD). Sociodemographic variables were collected with a standardized individual questionnaire. HD (score ≥8) and binge drinking (BD) (drinks on the same occasion ≥5) were detected with the Alcohol Use Disorders Identification Test. The 4 SNPs were genotyped by the polymerase chain reaction-restriction fragment length polymorphism method. Adjusted odds ratios (aORs) and 95% confidence intervals (CIs) were calculated using logistic regression analysis. The Bonferroni correction was used (considering the 4 SNPs studied). RESULTS: There were no significant differences in the frequencies of Arg370Cys, Arg272Gln, and Ile350Val polymorphisms between HD cases and controls. Otherwise, Arg/His genotype (rs1229984) in ADH1B gene showed a protective effect against HD (aOR = 0.36; 95% CI: 0.14 to 0.90) and BD (aOR = 0.49; 95% CI: 0.21 to 0.95). Nevertheless, these differences were no longer significant after Bonferroni correction. CONCLUSIONS: The results of this study suggest a possible effect of the Arg48His genotype on the protection against HD in HIV-1-positive individuals.

The impact of home fortification with multiple micronutrient powder on vitamin A status in young children: A multicenter pragmatic controlled trial in Brazil.

Home fortification with multiple micronutrient powder (MNP) is effective in the prevention of anemia in young children. However, the impact on their vitamin A status remains controversial. This study aimed to evaluate the effect of MNP on vitamin A status in young Brazilian children. A multicenter pragmatic, controlled trial was carried out in primary health centers in four Brazilian cities. In the beginning of the study, the control group (CG) consisted of children 11-14 months old (n = 395) attending in routine pediatric health care. In parallel, the intervention group (IG) was composed of children 6-8 months old (n = 399), in the same health centers, who followed the intervention with MNP for 2-3 months. The analysis of the effect of MNP on vitamin A status was performed by comparing the IG with the CG after a 4- to 6-month follow-up when IG children had reached the age of the controls. The prevalence of vitamin A deficiency (VAD; serum retinol <0.70 µmol/L) in the CG was 16.2%, while in the IG was 7.5%-a 55% reduction in the VAD [prevalence ratio (95% confidence interval) = 0.45 (0.28; 0.72)]. This reduction was also significant when stratifying the study centers by coverage of the Brazilian Vitamin A Supplementation Program. The adjusted mean of vitamin A serum concentrations improved in the IG compared with CG children, with a shift to the right in the vitamin A distribution. Home fortification with MNP was effective in reducing VAD among young Brazilian children.

Interferon lambda 4 (IFNL4) gene polymorphism is associated with spontaneous clearance of HCV in HIV-1 positive patients.

Approximately one-third of the individuals infected with human immunodeficiency virus type 1 (HIV-1) are co-infected with hepatitis C virus (HCV). Co-infected patients have an increased risk for developing end-stage liver diseases. Variants upstream of the IFNL3 gene have been associated with spontaneous and treatment-induced clearance of HCV infection. Recently, a novel polymorphism was discovered, denoted IFNL4 ΔG > TT (rs368234815), which seems to be a better predictor of spontaneous clearance than the IFNL4 rs12979860 polymorphism. We aimed to determine the prevalence of the IFNL4 ΔG > TT variants and to evaluate the association with spontaneous clearance of HCV infection in Brazilian HIV-1 patients. The IFNL4 ΔG > TT genotypes were analyzed by polymerase chain reaction followed by restriction digestion in 138 HIV-1 positive patients who had an anti-HCV positive result. Spontaneous clearance of HCV was observed in 34 individuals (24.6%). IFNL4 genotype distribution was significantly different between individuals who had spontaneous clearance and chronic HCV patients (p=0.002). The probability of spontaneous clearance of HCV infection for patients with the IFNL4 TT/TT genotype was 3.6 times higher than for patients carrying the IFNL4 ΔG allele (OR=3.63, 95% CI:1.51-8.89, p=0.001). The IFNL4 ΔG > TT polymorphism seems to be better than IFNL4 rs12979860 to predict spontaneous clearance of the HCV in Brazilian HIV-1 positive patients.

Short communication: Prevalence and risk factors for human T cell lymphotropic virus infection in Southern Brazilian HIV-positive patients.

HIV/human T cell lymphotropic virus (HTLV) coinfection has a large range of prevalence in the different risk groups and geographic regions of the world. Most of the HTLV-infected people live in geographic areas where the virus is endemic, as it happens in Brazil. The aim of this study was to identify HTLV prevalence and risk factors in HIV-positive patients. A cross-sectional study was conducted with 580 HIV-positive patients (mean age of 40.6 years and 45.0% men) from a specialized HIV/AIDS diagnosis and treatment center in Southern Brazil. Sociodemographic data, HIV risk factors, and HTLV-1/2 antibodies were collected. HTLV proviral DNA was detected by polymerase chain reaction (PCR). A multivariate analysis was performed to identify risk factors for HTLV infection. HTLV antibodies were detected in 29 (5.0%) and HTLV provirus in 17 (2.9%) patients. HTLV-1 was identified in 11 (64.7%) patients and HTLV-2 in 6 (35.3%) patients. No significant differences were observed between mono and coinfected patients in clinical characteristics regarding HIV/AIDS (time since HIV diagnosis, HIV viral load, lymphocytes CD4(+) count, and use of highly active antiretroviral therapy). Blood transfusion history was significantly associated with HIV/HTLV coinfection (p=0.039). Alcohol abuse was more prevalent in HTLV-positive (47.1%) than in HIV mono-infected patients (20.4%; p=0.008). Tattooing was the only risk factor independently associated with HIV/HTLV coinfection (p=0.035). This information contributes to an understanding of the epidemiology of HIV/HTLV coinfection in Brazil.

Prevalências de adesão à terapia antirretroviral e fatores associados em pacientes adultos de três centros urbanos do Sul do Brasil / Prevalence of adherence to antiretroviral therapy and associated factors in adult patients of three urban centers of southern Brazil

OBJETIVO: Conhecer a prevalência e os fatores associados à adesão a terapia antirretroviral (TARV) em pacientes adultos atendidos no Serviço de Assistência Especializada aos portadores do HIV/Aids de três municípios do RS. MÉTODO: Estudo de delineamento transversal com coleta de dados através de questionários de dados sociodemográficos e referente ao esquema de tratamento; auto relato de medicamentos tomados; avaliações de problemas relacionados ao álcool (AUDIT); de transtornos psiquiátricos menores (SRQ-20) e do estado clínico no momento da entrevista. RESULTADOS: Foram entrevistados 453 pacientes dos quais 79,2% foram aderentes à TARV. Os homens apresentaram uma prevalência de adesão à TARV 37% maior em relação às mulheres. Os pacientes com estado clínico leve e moderado aderiram 18% mais do que os com estado clínico grave. CONCLUSÕES: As políticas de prevenção ao HIV/Aids devem considerar que a epidemia afeta homens e mulheres de forma heterogênea e que isto deve ser levado em conta para mobilizar, no paciente, o tratamento adequado.(AU)

OBJECTIVE: To determine the prevalence and associated factors to adherence in antiretroviral therapy (HAART) of adult patients treated at the Division of Specialized Care for HIV/Aids (SAE) of three cities in RS. METHODS: Cross-sectional multicenter study with data collected by a socio-demographic questionnaire with questions relative to the treatment regimen, self-report of medication taken and of minor psychiatric disorder (SRQ20), questionnaire Alcohol Use Disorders Identification Test (AUDIT), and assessment of the clinical status at the time of the interview. RESULTS: A total of 453 adult patients with HIV/Aids and users of the SAE was interviewed and 79.2% were adherent to HAART. Men presented 37% more prevalence of adherence to HAART in relation to women and patients with low and moderate clinical status presented 18% times more prevalence of adherence to HAART in relation to patients with grave clinical status. CONCLUSIONS: Policies to prevent HIV/Aids must consider that the epidemy affects men and women unequally which should be understood to mobilize, in the patient, appropriate treatment.(AU)

IL28B polymorphism associated with spontaneous clearance of hepatitis C infection in a Southern Brazilian HIV type 1 population.

About one-third of people infected with human immunodeficiency virus-1 (HIV-1) are coinfected with hepatitis C virus (HCV) because of shared transmission routes. Studies report that HIV-1 complicates hepatitis C infection by increasing HCV viral load and reducing spontaneous clearance. Single nucleotide polymorphisms (SNPs) upstream of the IL28B gene have been associated with spontaneous and treatment-induced clearance of HCV infection. The aim of this study was to evaluate the association between the SNP rs12979860 of the IL28B gene and spontaneous clearance of HCV infection in a Brazilian HIV-1 population. The SNP was analyzed by polymerase chain reaction (PCR) followed by restriction digestion in 138 anti-HCV-positive patients. Spontaneous clearance was observed in 34 subjects (24.6%). Genotype distribution was significantly different between spontaneous clearance and HCV chronic patients. The CT/TT genotypes conferred a nearly 3-fold increased odds to chronic HCV infection relative to the CC genotype (odds ratio, 2.78; 95% confidence interval, 1.16-6.64; p=0.011). In conclusion, the rs12979860 polymorphism is associated with spontaneous clearance of HCV in HIV-1 Brazilian infected patients.

Prevalência de subtipos do HIV-1 em amostra de pacientes de um centro urbano no sul do Brasil / Prevalence of HIV-1 subtypes in patients of an urban center in Southern Brazil / Prevalencia de subtipos de VIH-1 en muestra de pacientes de un centro urbano en el sur de Brasil

OBJETIVO: Estimar a prevalência dos subtipos do HIV-1 e analisar fatores associados. MÉTODOS: Foi realizado um estudo transversal com amostra de conveniência de 80 pacientes adultos HIV-positivos atendidos em serviço especializado em DST/Aids em Canoas, RS, no período de julho de 2008 a janeiro de 2009. A determinação dos subtipos do HIV foi realizada por amplificação de fragmento do genoma viral pela reação em cadeia da polimerase seguida do seqüenciamento dos fragmentos amplificados. Variáveis sociodemográficas, clínicas e comportamentais foram coletadas em questionário estruturado. Foi realizada análise estatística univariada utilizando os testes de qui-quadrado e t de Student. RESULTADOS: Foi observada uma prevalência maior do subtipo C (43,8 por cento; IC 95 por cento: 32,9;54,6), seguida pelo CRF31_BC (35,0 por cento; IC 95 por cento: 24,6;45,5) e subtipos B (18,8 por cento; IC 95 por cento: 10,2;27,3) e F (2,4 por cento; IC 95 por cento: 0;5,9). Outros subtipos de HIV-1 não foram observados. Pacientes infectados com CRF31_BC apresentaram diagnóstico mais recente do que os pacientes infectados com o subtipo B (p < 0,05). Observou-se também maior freqüência de co-infecção com outros vírus (hepatites B e C e T-linfotrópicos humanos) nos indivíduos portadores do CRF31_BC do que nos demais subtipos. Com relação aos aspectos sociodemográficos, não foram observadas diferenças na distribuição dos subtipos e formas recombinantes quanto ao sexo e práticas sexuais. CONCLUSÕES: Os resultados obtidos indicam uma freqüência maior do subtipo C e do CRF31_BC nesse centro urbano do sul do Brasil, com possíveis vias de transmissão diferentes.

OBJECTIVE: To estimate the prevalence of HIV-1 subtypes and analyze factors associated. METHODS: A cross-sectional study was performed with a convenience sample of 80 adult HIV-positive patients, users of an AIDS/STD specialized service, in the city of Canoas, Southern Brazil, between July 2008 and January 2009. Determination of HIV subtypes was performed with the amplification of viral genome fragment, using polymerase chain reaction, followed by sequencing of the amplified fragments. Sociodemographic, clinical and behavioral variables were collected in a structured questionnaire. Univariate statistical analysis was performed, using chi-square test and Student's t-test. RESULTS: A higher prevalence of subtype C was found (43.8%; 95% CI: 32.9;54.6), followed by CRF31_BC (35.0%; 95% CI: 24.6;45.5) and subtypes B (18.8%; 95% CI: 10.2;27.3) and F (2.4%; 95% CI: 0;5.9). Other HIV-1 subtypes were not observed. Patients infected with CRF31_BC were diagnosed more recently than patients infected with subtype B (p<0.05). In addition, there was a higher frequency of co-infection with other viruses (hepatitis B and C and human T-lymphotropic viruses) in individuals with CRF31_BC, compared to other subtypes. With regard to sociodemographic aspects, there were no differences in the distribution of subtypes and recombinant forms, in terms of gender and sexual practices. CONCLUSIONS: Results obtained indicate a higher frequency of subtype C and CRF31_BC in this urban center of Southern Brazil, with possible different ways of transmission.

OBJETIVO: Estimar la prevalencia de los subtipos de VIH-1 y analizar factores asociados. MÉTODOS: Se realizó un estudio transversal con muestra de conveniencia de 80 pacientes adultos VIH-positivos atendidos en servicio especializado en DST/Sida en Canoas, Sur de Brasil, en el período de julio de 2008 a enero de 2009. La determinación de los subtipos de VIH fue realizada por amplificación de fragmento del genoma viral por la reacción en cadena de la polimerasa seguida de la secuenciación de los fragmentos amplificados. Variables sociodemográficas, clínicas y conductuales fueron colectadas en cuestionario estructurado. Se realizó análisis estadístico univariado utilizando las pruebas de chi-cuadrado y t de Student. RESULTADOS: Se observó una prevalencia mayor del subtipo C (43,8%; IC 95%:32,9;54,6), seguido de la CRF31_BC (35,0%; IC 95%: 24,6;45,5) y subtipos B (18,8%; IC 95%: 10,2;27,3) y F (2,4%; IC 95%: 0;5,9). Otros subtipos de VIH-1 no fueron observados. Pacientes infectados con CRF31_BC presentaron diagnóstico más reciente que los pacientes infectados con el subtipo B (p<0,05). Se observó también una mayor frecuencia de co-infección con otros virus (hepatitis B y C y T-linfotrópicos humanos) en los individuos portadores del CRF31_BC en comparación con los demás subtipos. Con relación a los aspectos sociodemográficos, no fueron observadas diferencias en la distribución de los subtipos y formas recombinantes con relación al sexo y prácticas sexuales. CONCLUSIONES: Los datos obtenidos en el presente estudio indican una mayor frecuencia del subtipo C y de la CRF31_BC en este centro urbano del sur de Brasil y sugieren que dichos subtipos pueden presentar vías de transmisión diferentes.

[Prevalence of HIV-1 subtypes in patients of an urban center in Southern Brazil]. / Prevalência de subtipos do HIV-1 em amostra de pacientes de um centro urbano no sul do Brasil.

OBJECTIVE: To estimate the prevalence of HIV-1 subtypes and analyze factors associated. METHODS: A cross-sectional study was performed with a convenience sample of 80 adult HIV-positive patients, users of an AIDS/STD specialized service, in the city of Canoas, Southern Brazil, between July 2008 and January 2009. Determination of HIV subtypes was performed with the amplification of viral genome fragment, using polymerase chain reaction, followed by sequencing of the amplified fragments. Sociodemographic, clinical and behavioral variables were collected in a structured questionnaire. Univariate statistical analysis was performed, using chi-square test and Student's t-test. RESULTS: A higher prevalence of subtype C was found (43.8%; 95% CI: 32.9;54.6), followed by CRF31_BC (35.0%; 95% CI: 24.6;45.5) and subtypes B (18.8%; 95% CI: 10.2;27.3) and F (2.4%; 95% CI: 0;5.9). Other HIV-1 subtypes were not observed. Patients infected with CRF31_BC were diagnosed more recently than patients infected with subtype B (p<0.05). In addition, there was a higher frequency of co-infection with other viruses (hepatitis B and C and human T-lymphotropic viruses) in individuals with CRF31_BC, compared to other subtypes. With regard to sociodemographic aspects, there were no differences in the distribution of subtypes and recombinant forms, in terms of gender and sexual practices. CONCLUSIONS: Results obtained indicate a higher frequency of subtype C and CRF31_BC in this urban center of Southern Brazil, with possible different ways of transmission.

Avaliação do índice de massa corporal da comunidade na IX semana da solidariedade da Pontifícia Universidade Católica do Rio Grande do Sul / Community evaluation of body mass index in the IX PUCRS Solidarity Week

Este trabalho tem por objetivo apresentar os resultados na comunidade que participou da Feira Comunitária na IX Semana da Solidariedade da Pontifícia Universidade Católica do Rio Grande do Sul/ 2003 a partir dos valores dos índices de massa corporal – IMC. Foram coletados dados antropométricos de peso e altura, realizado cálculo do IMC e classificado, segundo a Organização Mundial da Saúde. Foram avaliados 630 indivíduos de ambos os sexos, com idades entre 10 e 90 anos. Em relação ao sexo masculino, observa-se nas faixas etárias de 51 a 90 valores de sobrepeso entre 47 a 66,7%. E os valores para obesidade entre 25 a 32%. Em relação ao sexo feminino, os valores encontrados para sobrepeso são de 38 a 50%. Os valores de obesidade atingem 30% desta população. Para a obesidade mórbida, em relação ao total da população avaliada dividida por sexo, esses valores representam 1,5 e 3,84%, respectivamente. Iniciativas como a das feiras de saúde tem função social importante, auxiliando no diagnóstico destes agravos nutricionais e trabalhando os aspectos preventivos, visando a promoção e educação em saúde para a comunidade em geral.